Volume-2 ,Issue-4, April-2026

The present study involves the physicochemical characterization and UV spectrophotometric method validation of Aceclofenac and Thiocolchicoside. Physically, Aceclofenac was observed as a white to off-white, odorless, fine crystalline powder, while Thiocolchicoside appeared as a yellow, odorless crystalline powder. Solubility studies indicated both drugs were soluble in methanol and DMSO, with poor solubility in water, making methanol a suitable solvent for analysis. pH values were 6.8 and 7.2, respectively, indicating stability under near-neutral conditions. Melting point results (150°C for Aceclofenac and 197°C for Thiocolchicoside) confirmed sample purity. UV analysis identified λ max at 278.0 nm and 369.0 nm for Aceclofenac and Thiocolchicoside, respectively, with linear calibration curves across their concentration ranges (R² = 0.998 and 0.999). FTIR analysis confirmed the presence of key functional groups. Method validation demonstrated excellent precision, accuracy, ruggedness, and robustness, with low %RSD values. LOD and LOQ were within acceptable limits, confirming the method's sensitivity and reliability for routine quantitative analysis.

Keyword: Aceclofenac, Thiocolchicoside, UV spectroscopy, method validation, calibration curve, FTIR, solubility, precision, LOD, LOQ